Education
Licence en Sciences Chimiques, Université de Liège, Liège, Belgium
M.S. in Chemistry, Carnegie-Mellon University, Pittsburgh
Ph.D. in Chemistry, Carnegie-Mellon University, Pittsburgh (1982)
Postdoctoral research in Chemistry, University of California, Davis
Professional Experience
3/87 – 6/93: The Pennsylvania State University; Assistant Professor of Chemistry
7/93 – 6/07: The Pennsylvania State University; Associate Professor of Chemistry
7/07 – 8/07: The Pennsylvania State University; Professor of Chemistry
9/07 – present: Johns Hopkins University; Professor of Biophysics
Displaying 15 recent publications.
Johnson, E.A., Russo, M.M., Nye, D.B., Schlessman, J.L.,and Lecomte J.T.J. (2018) Lysine as a Heme Iron Ligand: A Property Common to Three Truncated Hemoglobins from Chlamydomonas reinhardtii. Biochimica et Biophysica Acta – General Subjects 1862:2660–2673. doi:10.1016/j.bbagen.2018.08.009
Nye, D.B. and Lecomte J.T.J. (2018) Replacement of the Distal Histidine Reveals a Non-Canonical Heme Binding Site in a 2-on-2 Hemoglobin. Biochemistry 57:5785–5796. doi:10.1021/acs.biochem.8b00752
Nye, D.B., Preimesberger, M.R., Majumdar, A., and Lecomte J.T.J. (2018) Histidine-Lysine Axial Ligand Switching in a Hemoglobin: A Role for Heme Propionates. Biochemistry 57:631–644. doi:10.1021/acs.biochem.7b01155
Preimesberger, M.R., Johnson, E.A., Nye, D.B., and Lecomte J.T.J. (2017) Covalent Attachment of the Heme to Synechococcus Hemoglobin Alters Its Reactivity Toward Nitric Oxide. Journal of Inorganic Biochemistry 177:171–182. doi:10.1016/j.jinorgbio.2017.09.018
Preimesberger, M.R., Majumdar, and Lecomte, J.T.J. (2017) Dynamics of Lysine as a Heme Axial Ligand: NMR Analysis of the Chlamydomonas reinhardtii Hemoglobin THB1. Biochemistry 56:551–569. doi:10.1021/acs.biochem.6b00926
Johnson, E.A. and Lecomte J.T.J. (2015) The Haemoglobins of Algae. Advances in Microbial Physiology 67:177–234. doi:10.1016/bs.ampbs.2015.08.003
Preimesberger, M.R., Majumdar, A., Rice, S.L., Que, L., and Lecomte, J.T.J. (2015) Helix-capping histidines: Diversity of N-H...N Hydrogen Bond Strength Revealed by 2hJNN Scalar Couplings. Biochemistry, 54:6896–6908. doi:10.1021/acs.biochem.5b01002
Rice, S.L., Boucher, L.E., Schlessman, J.L., Preimesberger, M.R., Bosch, J., and Lecomte, J.T.J. (2015) Structure of Chlamydomonas reinhardtii THB1, a Group 1 Truncated Hemoglobin with a Rare Histidine-Lysine Heme Ligation. Acta Crystallographica Section F Structural Biology Communications 71:718–725. doi:10.1107/S2053230X15006949
Preimesberger, M.R., Majumdar, A., Aksel, T., Sforza, K., Lectka, T., Barrick, D., and Lecomte, J.T.J. (2015) Direct NMR Detection of Bifurcated Hydrogen Bonding in the α-Helix N-Caps of Ankyrin Repeat Proteins. Journal of the American Chemical Society 137:1008–1011. doi:10.1021/ja510784g
Johnson, E.A. and Lecomte J.T.J. (2014) Characterization of the Truncated Hemoglobin THB1 from Protein Extracts of Chlamydomonas reinhardtii. F1000Research 3:294. doi:10.12688/f1000research.5873.1
Rice, S.L., Preimesberger, M.R., Johnson, E.A., and Lecomte, J.T.J. (2014) Introduction of a Covalent Histidine–Heme Linkage in a Hemoglobin: A Promising Tool for Heme Protein Engineering. Journal of Inorganic Biochemistry 141:198–207. doi:10.1016/j.jinorgbio.2014.09.009
Johnson, E.A., Rice, S.L., Preimesberger, M.R., Nye, D.B., Gilevicius, L., Wenke, B.B., Brown, J.M., Witman, G.B., and Lecomte, J.T.J. (2014) THB1, a Truncated Hemoglobin with Lysine as a Distal Heme Ligand, Is Linked to Nitrogen Metabolism in Chlamydomonas reinhardtii. Biochemistry 53:4573–4589.
Wenke, B.B., Lecomte, J.T.J., Héroux, A., and Schlessman, J.L. (2014) The 2/2 Hemoglobin from the Cyanobacterium Synechococcus sp. PCC 7002 with Covalently Attached Heme: Comparison of X-ray and NMR Structures. Proteins 82:528–534. doi:10.1002/prot.24409
Preimesberger, M.R., Wenke, B.B., Gilevicius, L., Pond, M.P., and Lecomte, J.T.J. (2013) Covalent Heme Attachment in Synechocystis Hemoglobin: Implications for Facile Histidine Posttranslational Modification. Biochemistry 42:3478–3488. doi:10.1021/bi400289e
Dellarole, M., Roumestand, C., Royer, C., and Lecomte, J.T.J. (2013) Volumetric Properties Underlying Ligand Binding In a Monomeric Hemoglobin: A High-Pressure NMR Study. Biochimica et Biophysica Acta 1834:1910–1922. doi:10.1016/j.bbapap.2013.04.016
Pond, M.P., Majumdar, A., and Lecomte, J.T.J. (2012) Influence of Heme Post-Translational Modification and Distal Ligation on the Backbone Dynamics of a Monomeric Hemoglobin. Biochemistry 51:5733–5747. PMID: 22775272 doi:10.1021/bi300624a