Juliette Lecomte

Professor

116 Jenkins Hall
410-516-7019
410-516-4118
lecomte_jtj@jhu.edu
Group/Lab Website

Biography
Research
Publications
Education

Licence en Sciences Chimiques, Université de Liège, Liège, Belgium

M.S. in Chemistry, Carnegie-Mellon University, Pittsburgh

Ph.D. in Chemistry, Carnegie-Mellon University, Pittsburgh (1982)

Postdoctoral research in Chemistry, University of California, Davis

Professional Experience

3/87 – 6/93: The Pennsylvania State University; Assistant Professor of Chemistry

7/93 – 6/07: The Pennsylvania State University; Associate Professor of Chemistry

7/07 – 8/07: The Pennsylvania State University; Professor of Chemistry

9/07 – present: Johns Hopkins University; Professor of Biophysics

The Lecomte group is interested in the structural, functional, and evolutionary properties of heme proteins. The main experimental technique used for the studies is nuclear magnetic resonance spectroscopy.

Displaying 15 recent publications. Full list available here.

Johnson, E.A. and Lecomte J.T.J. (2015) The Haemoglobins of Algae. Advances in Microbial Physiology 67:177–234. doi:10.1016/bs.ampbs.2015.08.003

Preimesberger, M.R., Majumdar, A., Rice, S.L., Que, L., and Lecomte, J.T.J. (2015) Helix-capping histidines: Diversity of N-H...N Hydrogen Bond Strength Revealed by 2hJNN Scalar Couplings. Biochemistry, 54:6896–6908. doi:10.1021/acs.biochem.5b01002

Rice, S.L., Boucher, L.E., Schlessman, J.L., Preimesberger, M.R., Bosch, J., and Lecomte, J.T.J. (2015) Structure of Chlamydomonas reinhardtii THB1, a Group 1 Truncated Hemoglobin with a Rare Histidine-Lysine Heme Ligation. Acta Crystallographica Section F Structural Biology Communications 71:718–725. doi:10.1107/S2053230X15006949

Preimesberger, M.R., Majumdar, A., Aksel, T., Sforza, K., Lectka, T., Barrick, D., and Lecomte, J.T.J. (2015) Direct NMR Detection of Bifurcated Hydrogen Bonding in the α-Helix N-Caps of Ankyrin Repeat Proteins. Journal of the American Chemical Society 137:1008–1011. doi:10.1021/ja510784g

Johnson, E.A. and Lecomte J.T.J. (2014) Characterization of the Truncated Hemoglobin THB1 from Protein Extracts of Chlamydomonas reinhardtii. F1000Research 3:294. doi:10.12688/f1000research.5873.1

Rice, S.L., Preimesberger, M.R., Johnson, E.A., and Lecomte, J.T.J. (2014) Introduction of a Covalent Histidine–Heme Linkage in a Hemoglobin: A Promising Tool for Heme Protein Engineering. Journal of Inorganic Biochemistry 141:198–207. doi:10.1016/j.jinorgbio.2014.09.009

Johnson, E.A., Rice, S.L., Preimesberger, M.R., Nye, D.B., Gilevicius, L., Wenke, B.B., Brown, J.M., Witman, G.B., and Lecomte, J.T.J. (2014) THB1, a Truncated Hemoglobin with Lysine as a Distal Heme Ligand, Is Linked to Nitrogen Metabolism in Chlamydomonas reinhardtii. Biochemistry 53:4573–4589.

Wenke, B.B., Lecomte, J.T.J., Héroux, A., and Schlessman, J.L. (2014) The 2/2 Hemoglobin from the Cyanobacterium Synechococcus sp. PCC 7002 with Covalently Attached Heme: Comparison of X-ray and NMR Structures. Proteins 82:528–534. doi:10.1002/prot.24409

Preimesberger, M.R., Wenke, B.B., Gilevicius, L., Pond, M.P., and Lecomte, J.T.J. (2013) Covalent Heme Attachment in Synechocystis Hemoglobin: Implications for Facile Histidine Posttranslational Modification. Biochemistry 42:3478–3488. doi:10.1021/bi400289e

Dellarole, M., Roumestand, C., Royer, C., and Lecomte, J.T.J. (2013) Volumetric Properties Underlying Ligand Binding In a Monomeric Hemoglobin: A High-Pressure NMR Study. Biochimica et Biophysica Acta 1834:1910–1922. doi:10.1016/j.bbapap.2013.04.016

Pond, M.P., Majumdar, A., and Lecomte, J.T.J. (2012) Influence of Heme Post-Translational Modification and Distal Ligation on the Backbone Dynamics of a Monomeric Hemoglobin. Biochemistry 51:5733–5747. PMID: 22775272 doi:10.1021/bi300624a

Pond, M.P., Wenke, B.B., Preimesberger, M.R., Rice, S.L., and Lecomte, J.T.J. (2012) 3-Fluorotyrosine as a Complementary Probe of Hemoglobin Structure and Dynamics: A 19F NMR Study of Synechococcus sp. PCC 7002 GlbN. Chemistry and Biodiversity 9:1703–1717. doi:10.1002/cbdv.201100448

Preimesberger, M.R., Pond, M.P., Majumdar, A., and Lecomte, J.T.J. (2012) Electron Self-Exchange and Self-Amplified Post-Translational Modification in the Hemoglobins from Synechocystis sp. PCC 6803 and Synechococcus sp. PCC 7002. Journal of Biological Inorganic Chemistry 17:599–609. doi:10.1007/s00775-012-0880-5

Nothnagel, H.J., Winer, B.Y., Vuletich, D.A., Pond, M.P., and Lecomte, J.T.J. (2011) Structural Properties of 2/2 Hemoglobins: The Group III Protein from Helicobacter hepaticus. IUBMB Life 63:197–205. doi:10.1002/iub.430

Nothnagel, H.J., Preimesberger, M.R., Pond, M.P., Winer, B.Y., Adney, E.A., and Lecomte, J.T.J. (2011) Chemical Reactivity of Synechococcus sp. PCC 7002 and Synechocystis sp. PCC 6803 Hemoglobins: Covalent Heme Attachment and Bishistidine Coordination. Journal of Biological Inorganic Chemistry 16:539–552. doi:10.1007/s00775-011-0754-2