Sua Myong

Associate Professor

121A Mergenthaler Hall
410-516-5122
smyong1@jhu.edu
Group/Lab Website

Biography
Research
Publications

Sua Myong is an associate professor in the Department of Biophysics. Her research focuses on quantitative analysis of gene expression in single molecule and single cell.

Quantitative analysis of gene expression in single molecule and single cell

Our research is focused on dissecting biological pathways that control and modulate gene expression profiles that are pertinent to human diseases. We develop single molecule and single cell platforms to examine potential rate-limiting steps that contribute to modulating transcription and translation. In particular, we investigate RNA interference pathway and G-quadruplex DNA mediated promoter activity. In collaboration, we are also studying telomeric DNA processing and chromatin remodeling. Together, we seek to shed light on molecular orchestration and mechanism that govern the Central Dogma of Biology.

2016

Koh H., Wang X., Myong, S., “Visualizing repetitive diffusion activity of double-strand RNA binding proteins by single molecule fluorescence assays”, Methods 2016 Mar 21 pii: S1046-2023(16)30043-3. ( pdf )

Kim M., Kreig A., Lee C., Rube T., Calver J., Song J., Myong S., "Quantitative analysis and prediction of G-quadruplex forming sequences in double-stranded DNA", Nucleic Acid Research 2016 ( in press )

2015

Kreig, A., Calvert, J., Sanoica, J., Cullum, E., Tippana, R., Myong, S., “ G-quadruplex Formation in Double Strand DNA probed by NMM and CV Fluorescence”, Nucleic Acid Research 2015 Sep 18;43(16):7961-70. ( pdf )

Wang, X., Vukovic, L., Koh, H., Schulten, K., Myong, S., “Dynamic profiling of double-stranded RNA binding proteins”,Nucleic Acid Research 2015 Sep 3;43(15):7566-76. ( pdf )

Bell R.J.A., Rube H.T., Kreig A., Mancini A., Fouse S.F., Nagarajan R.P., Choi S., Hong C., He D., Pekmezci M., Wiencke J.K., Wrensch M.R., Chang S.M., Walsh K.M., Myong S., Song J.S., Costello J.F., “The transcription factor GABP selectively binds and activates the mutant TERT promoter in cancer” Science 2015 May 29;348(6238):1036-9. (pdf )

Elbaum-Garfinkle S., Kim Y., Szczepaniak C., Eckmann C., Myong S., Brangwynne CP., “The disordered P granule protein LAF-1 drives phase separation into droplets with tunable viscosity and dynamics” PNAS 2015 Jun 9;112(23):7189-94. ( pdf )

Qiu Y., Niu H., Vukovic L., Sung P., Myong S., “Molecular Mechanism of Resolving Trinucleotide Repeat Hairpin by Helicases” Structure 2015 Jun 2;23(6):1018-27. ( pdf )

Shim J., Kim Y., Humphreys GI., Nardulli AM., Kosari F, Vasmatzis G, Taylor WR., Ahlquist DA., Myong S, Bashir R., “Nanopore-Based Assay for Detection of Methylation in Double-Stranded DNA Fragments” ACS Nano 2015 Jan 27;9(1):290-300. ( pdf )

2014

Hwang, H., Opresko, PL., Myong, S., “Single-molecule real-time detection of telomerase extension activity” Scientific Reports 2014 Sep 29;4:6391. ( pdf )

Koh H., Xing L., Kleiman L., Myong S., “Repetitive RNA unwinding by RNA helicase A facilitates RNA annealing” Nucleic Acid Research 2014 Jul;42(13):8556-64. ( pdf )

Tippana R., Xiao W., Myong S., “G-quadruplex conformation and dynamics are determined by loop length and sequence”Nucleic Acid Research 2014 Jul;42(12):8106-14. ( pdf )

Hwang H., Kreig A., Calvert J., Lormand J., Kwon Y., Daley JM., Sung P., Opresko PL., Myong S., "Telomeric Overhang Length Determines Structural Dynamics and Accessibility to Telomerase and ALT-Associated Proteins" Structure 2014 Jun 10;22(6):842-53. ( pdf ): Highlight Preview Hopfner, K-P., “Single Molecule Choreography between Telomere Proteins and G Quadruplexes” Structure. 2014 Jun 10;22(6):801-2.

Vukovic L., Koh H., Myong S., Schulten K. “Substrate recognition and specificity of double-stranded RNA binding proteins” Biochemistry 2014 Jun 3;53(21):3457-66. ( pdf )

Lytle A. K., Origanti S. S., Qiu Y., VonGermeten J., Myong S. and Anthony E. "Context dependent remodeling of Rad51-DNA complexes by Srs2 is mediated by a specific protein-protein interaction" J. Mol. Biol. 2014 May 1;426(9):1883-97. (pdf )

Hwang H., and Myong S. “Protein induced fluorescence enhancement (PIFE) for probing protein–nucleic acid interactions” Chem. Soc. Rev., 2014 Feb 21;43(4):1221-9. ( pdf )

Qiu Y., and Myong S. "Single Molecule Imaging of proteins that recognize and repair DNA damages" Selected Topics in Quantum Electronics, IEEE Journal of , vol.20, no.2, pp.223,231, March-April 2014. ( pdf )

2013

Qiu Y., Antony E., Doganay S., Koh H., Lohman T. M., and Myong S. "Srs2 prevents Rad51 filament formation by repetitive motion on DNA". Nat Commun. 2013 Aug 13;4:2281. ( pdf )

Bruns A.M., Pollpeter D., Hadizadeh N., Myong S., Marko J.F., Horvath C.M., "ATP Hydrolysis Enhances RNA Recognition and Antiviral Signal Transduction by the Innate Immune Sensor", Laboratory of Genetics and Physiology 2 (LGP2). J Biol Chem. 2013 Jan 11;288(2):938-46. ( pdf )

Koh H., Kidwell M., Ragunathan K., Doudna J., and Myong S., "ATP independent diffusion of double stranded RNA binding protein". Proc Natl Acad Sci. 2013 Jan 2;110(1):151-6. ( pdf )

2012

Hwang H., Buncher N., Opresko P. L., and Myong S., "POT1-TPP1 regulates telomeric overhang structural dynamics".Structure. 2012 Nov 7;20(11):1872-80. ( pdf )

Jia H., Korolev S., Niedziela-Majka A., Maluf N. K., Gauss G.H., Myong S., Ha T., Waksman G., Lohman T.M. "Rotations of the 2B sub-domain of E. coli UvrD helicase/translocase coupled to nucleotide and DNA binding". J Mol Biol. 2011 Aug 19;411(3):633-48. Erratum in: J Mol Biol. 2012 May 4;418(3-4):264-5. ( pdf )

2011

Torbeev, V. Yu., Myong, S., Ha, T. and Kent, S. B. H. “Single-Molecule Studies of HIV-1 Protease Catalysis Enabled by Chemical Protein Synthesis” Isr. J. Chem. 51: 960–967. November 2011 ( pdf )

Ha T, Myong S. "A single-molecule view of chaperonin cooperativity". Proc Natl Acad Sci. U S A. 2011 Oct 11;108(41):16865-6. ( pdf )

Balci H., Arslan S., Myong S., Lohman T.M., Ha T. "Single-molecule nanopositioning: structural transitions of a helicase-DNA complex during ATP hydrolysis". Biophys J. 2011 Aug 17;101(4):976-84. ( pdf )

Hwang H., Kim H. and Myong S. "Protein induced fluorescence enhancement as a single molecule assay with short distance sensitivity" Proc Natl Acad Sci. 108(18):7414-8 (2011) ( pdf )

Qiu Y. and Myong S. "Single molecule detection of one, two and multiplex proteins involved in DNA/RNA transaction"Cellular and Molecular Bioengineering 4, 2, 125-137 (2011) ( pdf )

BEFORE 2011

Park J., Myong S., Niedziela-Majka A., Yu J., Lohman T. M. and Ha T."Reeling in DNA One Base at a Time: PcrA translocation coupled to DNA looping dismantles RecA filaments" Cell 142, 4, 544-555, (2010) ( pdf )

Myong, S. and Ha T., "Stepwise translocation of nucleic acid motors", Current Opinion in Structural Biology 20 (1), 121-127 (2010) ( pdf )

Okumus, B., Arslan S., Fengler S., Myong S. and Ha T., "Single Molecule Nanocontainers Made Porous Using a Bacterial Toxin", J. Am. Chem. Soc. 131, 14844–14849 (2009) ( pdf )

Myong, S., Cui S., Cornish P. V., Kirchhofer A. , Gack M. U., Jung J. U., Hopfner K. P. and Ha T., "Cytosolic viral sensor RIG-I is a 5 prime - triphosphate-dependent translocase on double-stranded RNA", Science 323(5917), 1070-1074 (2009) ( pdf )

Gack, M. U., Kirchhofer A., Shin Y. C., Inn K. S., Liang C., Cui S., Myong S., Ha T., Hopfner K. P. and Jung J. U., "Roles of RIG-I N-terminal tandem CARD and splice variant in TRIM25-mediated anti-viral signal transduction", Proc Natl Acad Sci, USA 105(43), 16743-16748 (2008) ( pdf )

Myong, S., Bruno M. M., Pyle A. M. and Ha T., "Spring-loaded Mechanism of DNA Unwinding by HCV NS3 Helicase",Science 317, 513-516 (2007) ( pdf )

Rasnik, I., Myong S., and Ha T., "Unraveling Helicase Mechanisms One Molecule at a Time", Nucleic Acid Research 34, 4225-4231 (2006) ( pdf )

Joo, C., McKinney S. A., Nakamura M., Rasnik I., Myong S., and Ha T., "Real Time Observation of RecA Filament Dynamics with Single Monomer Resolution", Cell 126, 515-527 (2006) ( pdf )

Myong, S., Stevens B., and Ha T., "Bridging Conformational Dynamics and Function using Single-Molecule Spectroscopy", Structure 14, 633-643 (2006) ( pdf )

Myong, S., Rasnik I., Joo C., Lohman T. M., and Ha T., "Repetitive Shuttling of a Motor Protein on DNA", Nature 437, 1321-1325 (2005) ( pdf )

Rasnik, I., Myong S., Cheng W., Lohman T. M., and Ha T., "DNA-binding Orientation and Domain Conformation of the E. Coli Rep Helicase Monomer Bound to a Partial Duplex Junction: Single-molecule Studies of Fluorescently Labeled Enzymes", J. of Mol. Biology 336(2), 395-408 (2004) ( pdf )